Our lab has a long‑standing interest in the interplay between nuclear organization and genome function.
Over the years, our team has developed strong expertise in molecular approaches including next-generation-sequencing (NGS)-based approaches (ChIP-seq, RNA-seq, etc.), live-cell imaging and more recently single-molecule microscopy (PALM: Photoactivated localization microscopy).
As a model to study cell-to-cell heterogeneity, our lab studies budding yeast during quiescence and upon return to growth.
Quiescence ensures survival in the face of unfavorable conditions through the establishment of a reversible, non-growing state, a property involved in the persistence of pathogens in their host. The genome of budding yeast quiescent cells adopts a very specific conformation associated with a massive decrease in transcriptional activity, two features that contribute to their longevity (Guidi et al, 2015; McKnight et al, 2015). Return to growth is a key step for survival in a competitive environment and requires (re)establishing the transcriptional program adapted to the available resources. Combining genetics with super-resolution microscopy (PALM), chromatin profiling (ChIp-seq) and transcriptome analysis will allow decipheromg the mechanisms leading to genome reactivation in relation to chromatin unfolding.
Following genome reactivation at the single-cell level using multiplex RNA-FISH (in collaboration with the Bienko lab), our lab wants to assess the contribution of cell to cell heterogeneity in helping the population to adapt to unpredictable conditions.
Keywords & model systems
chromatin, epigenetics, nuclear architecture, nuclear compartments
Saccharomyces cerevisiae (budding yeast)
|since 2012||Research Director CNRS||CNRS, France|
|since 2015||Head of the Research Unit Nuclear Dynamics CNRS-Institut Curie||Institut Curie, Paris, France|
|2007-2012||Junior group leader||Institut Curie, Paris, France|
|2004-2006||Post-doctoral fellow||Friedrich Miescher Institute (FMI), Basel, Switzerland|
|2001-2003||EMBO Post-doctoral fellow||University of Geneva, Switzerland|
|2000||PhD||Université Pierre et Marie Curie, Paris, France|
This project has received funding from the European Union’s Horizon 2020 Research and Innovation programme under the Marie Skłodowska-Curie grant number 860675.